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When white blood cells are removed may affect stored blood quality

NCT ID NCT07821736

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First seen Sep 16, 2026 · Last updated Sep 17, 2026 · Updated 1 time

Summary

Researchers at Assiut University are studying how the timing of leukoreduction, the removal of white blood cells from donated blood, affects levels of a marker called H3cit and the quality of stored red blood cells. The study uses 60 standard whole blood units from healthy donors. Scientists measure H3cit at several points during storage and compare it with standard blood storage markers to see whether filtration timing matters.

What this could mean

Our plain-language read of the trial. This is informational only, not medical advice or a prediction.

Active substance
Timing of leukoreduction (white blood cell removal) during blood processing
What this could lead to
If the timing of leukoreduction affects H3cit levels, blood banks could adjust their processing schedules to reduce storage lesions and improve the quality of stored red blood cells for transfusion.
What could go wrong
This is a small laboratory study of 60 blood units, not a clinical trial in patients. It may find no meaningful link between leukoreduction timing and H3cit, and any finding would need confirmation in larger studies before changing blood bank practices.

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Study facts

What this study's own registry entry says, in plain language.

Phase

Not a phased trial

Phase numbers describe drug development. The registry uses this when they do not apply, as it does for trials of devices, procedures or behaviour changes, and for observational studies.

Participants

About 60 people

The number the study aims to enrol. It can still change while the study runs.

Expected to start

Nov 2026

An estimate. Start dates often move.

Expected to finish

Dec 2028

An estimate. End dates often move.

Lead sponsor

Other sponsor

The registry's catch-all category, for sponsors it does not file as a company, a government agency, or a research network.

Who can take part

This study's own entry requirements. Only the study team can say for certain whether you qualify.

Ages

18 to 60 years

Sex

Anyone

Healthy volunteers

Accepted

You do not need to have the condition being studied to take part.

Show the full entry requirements

Copied word for word from the study's registry entry, so the wording is the study team's rather than ours.

Inclusion Criteria: * • Standard whole blood units collected from healthy voluntary donors who meet the national blood transfusion service fitness criteria (age 18-60 years, body weight ≥50 kg). * Collection Volume \& Container: Standard unit volume (450 ± 45 mL) collected into closed bag systems with additive/anticoagulant solution (CPDA-1). * Thermal Storage Chain: Blood units maintained continuously within standard blood bank temperature limits (2 C to 6C )from collection until the end of the storage period. Baseline Hematological Parameters: Normal baseline hematological profile immediately post-collection (Hemoglobin ≥12.5g dl) Exclusion Criteria: * Donor Clinical Factors: Donors with a history of acute or chronic inflammatory conditions, active infections, autoimmune diseases, or those who ingested anti-inflammatory/antiplatelet medications (e.g., NSAIDs, aspirin) within 14 days prior to donation. * Technical Collection Complications: Phlebotomy draw time exceeding 12 minutes, difficult venipuncture, or units displaying macro-clots or excessive lipemia post-collection. * Storage Excursions \& Bag Damage: Any blood unit subjected to cold-chain temperature violations outside 2-6C, physical damage, or system breaches/leaks. Transfusion-Transmitted Infections (TTI): Units testing reactive for screening markers, including HBsAg, Anti-HCV, Anti-HIV-1/2, or Syphilis * Donor Clinical Factors: Donors with a history of acute or chronic inflammatory conditions, active infections, autoimmune diseases, or those who ingested anti-inflammatory/antiplatelet medications (e.g., NSAIDs, aspirin) within 14 days prior to donation.

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Contacts and locations

Locations

  • Mayar Medhat

    Asyut, Egypt

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